2008 Weinstein Conference Schedule
Thursday May 15th 2008
10 AM– 4 PM: Registration (Ballroom Foyer)
1:45 PM: Opening Remarks-Jim Martin (AB Ballroom)
2:00- 3:05 PM: Platform Session I: Cardiomyocyte Biology
(Chairs: Aarif Khakoo and Brad Amendt) (AB Ballroom)
2:00- 2:05 Chair’s introduction
2:05- 2:20 Talk 1
ndrg4 is required for normal myocyte proliferation during early cardiac development and is downstream of tbx5 activation in zebrafish
Xianghu Qu1,2, Haibo Jia2,3, fDeborah. M. Garrity4, Kevin Tompkins1, Lorene Batts1, Bruce Appel2,5, Tao P. Zhong2,3, H. Scott Baldwin1,2
1Department of Pediatric Cardiology, 2Department of Cell and Developmental Biology, 3Department of Cardiovascular Medicine, 5Department of Biological Sciences, Vanderbilt University Medical Center, Nashville, TN 37232, USA. 4Department of Biology, Colorado State University, Fort Collins, CO, 80523, USA.
2:20- 2:35 Talk 2
A genetic interaction between Hopx and Hdac2 regulates cardiac development and myocyte proliferation
Chinmay M. Trivedi, Qiaohong Wang, Min Min Lu, Nicole Antonucci, and Jonathan A. Epstein.
Department of Cell and Developmental Biology, Penn Cardiovascular Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania-19104, USA.
2:35- 2:50 Talk 3
Tropomyosin is Required for Cardiac Myofibril Assembly, Cardiac Development, and Viability in the Mouse
Caroline R. McKeown and Velia M. Fowler
Department of Cell Biology, The Scripps Research Institute
2:50-3:05 Talk 4
Vertebrate heart growth is regulated by functional antagonism between Gridlock and Gata5
Haibo Jia*, Isabelle N. King, Sameer S. Chopra*, Haiyan Wan*, Terri T. Ni*, Charlie Jiang*, Xiaoqun Guan*, Sam Wells*, Deepak Srivastava, and Tao P. Zhong*
*Departments of Medicine and Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232; and Gladstone Institute of Cardiovascular Disease, Department of Pediatrics, University of California, San Francisco, CA 94158
3:05- 3:20 PM: Break
3:20- 4:25 PM: Platform Session II – Vascular Development
(Chairs: Mary Dickinson and Karen Hirschi) (AB Ballroom)
3:20- 3:25 Chair’s Introduction
3:25- 3:40 Talk 1
Loss of TGF-beta2 leads to aortic aneurysm during development
AZHAR, MOHAMAD
BIO5 Institute and Department of Cell Biology & Anatomy, University of Arizona
MRB 322, 1656 E Mabel St, PO Box 245217, Tucson, AZ 85724
3:40- 3:55 Talk 2
Temporal requirement of Pax3 in the cardiac neural crest cell lineage
Michael Olaopa*, Paige Snider, Anne Moon1 & Simon J Conway.
Cardiovascular Development Group, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 1Departments of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112.
3:55- 4:10 Talk 3
Ectoderm-derived Gbx2 acts downstream of Tbx1 to control a Slit/Robo-mediated neural crest cell migration pathway during arch artery development.
Amélie Calmont1¥, Sarah Ivins1¥, Kelly Lammerts Van Bueren1, Irinna Papangeli1, Vanessa kyriakopoulou1, Catherine Roberts1, M. Albert Basson2, Elizabeth A. Lindsay3, Antonio Baldini3 and Peter J. Scambler1¥, Equal contribution
1Molecular Medicine Unit, Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK
2Department of Cranofacial Development, King’s College London, Floor 27, Guy’s Tower, London SE1 9RT, UK
3Telethon Institute of Genetics and Medicine (Tigem), Via Pietro Castellino 111, Napoli 1-80131, Italy
4:10- 4:25 Talk 4
The role of the notochord in dorsal aortae fusion
Robert J. Garriock and Takashi Mikawa
University of California, San Francisco
4:25- 4:40 PM: Break
4:40- 5:45 PM: Platform Session III – SHF I
(Chairs: Robert Kelly and Nigel Brown) (A/B Ballroom)
4:40- 4:45 Chair’s Introduction
4:45- 5:00 Talk 1
An Fgf autocrine loop initiated in second heart field mesoderm regulates morphogenesis at the arterial pole of the heart
Eon Joo Park1, Yusuke Watanabe2, Graham Smyth3, Sachiko Miyagawa-Tomita4, Erik N. Meyers3, John Klingensmith3, Todd Camenisch5, Margaret Buckingham2, Anne M. Moon1,6, 7
1Department of Neurobiology and Anatomy, University of Utah; 2Department of Developmental Biology, Pasteur Institute, Paris, France; 3Department of Cell Biology, Duke University Medical Center, Durham, NC; 4Department of Pediatric Cardiology, Tokyo Women's Medical University, Tokyo, Japan; 5Department of Pharmacology & Toxicology, University of Arizona, Tuscon;
6Department of Pediatrics, and 7Program in Human Molecular Biology and Genetics, University of Utah, Salt Lake City
5:00- 5:15 Talk 2
Spatiotemporally specific FRS2a-mediated signals in cardiac outflow tract morphogenesis
Jue Zhang, Yongshun Lin, Yongyou Zhang, Yongsheng Lan, Chunhong Lin, Robert J. Schwartz1, James F. Martin, and Fen Wang
Center for Cancer and Stem Cell Biology, 1Center for Molecular Development and Disease, Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Blvd., Houston, TX 77030-3303.
5:15- 5:30 Talk 3
A precursor pool spatially and genetically distinct from the first and second heart fields contributes to the myocardium of the sinus venosus
M.T.M. Mommersteeg (1), J.N. Dominguez (2), J.B.E. Burch (3), N.A. Brown (2), A.F.M. Moorman (1), V.M. Christoffels (1)
1. Heart Failure Research Center, Academic Medical Center, Amsterdam, The Netherlands.
2. Division of Basic Medical Sciences, St George’s, University of London, London, UK.
3. Cell and Developmental Biology Program, Fox Chase Cancer Center, Philadelphia, USA.
5:30- 5:45 Talk 4
Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a novel Gata4-basedreporter of Cre activity
Qing Ma1^, Bin Zhou1^, and William Pu1*
1Department of Cardiology, Children’s Hospital Boston, 300 Longwood Ave, Boston, MA 02115
5:45- 6:00 PM: Break
6:00- 7:00 PM: Keynote Presentation - Eric Olson (AB Ballroom)
7:00- 10:00 PM: Reception (Liberty Hall)
7:00 – 9:00 PM: Poster Session 1 (even numbered posters are manned) (Liberty Hall)
Friday May 16th 2008
7:30- 9:00 AM: Continental Breakfast (Lamar)
9:00- 10:20 AM: Platform Session IV – Second Heart Field II
(Chairs: Karen Niederreither and Anne Moon) (AB Ballroom)
9:00-9:05 AM Chair’s Introduction
9:05- 9:20 Talk 1
INVESTIGATING BMP-SIGNALING FUNCTIONS IN SECOND HEART FIELD
Jun Wang*, Lijiang Ma*, Margarita Bonilla-Claudio*, and James F. Martin*
*Institute of Biosciences and Technology, Texas A&M University System Health Science Center, 2121 Holcombe Blvd, Houston, Texas, 77030
9:20- 9:35 Talk 2
Non-autonomous requirement for BMP signaling in morphogenesis of the cardiac outflow tract and right ventricle
Murim Choi1, Laura Barbosky1,2, Jianwen Que1, Naoki Mine1, Matthew Goddeeris1,2, Robert Schwartz3, Erik N. Meyers1,2, Margaret L. Kirby1,2, and John Klingensmith1,2, #Departments of Cell Biology (1) and Pediatrics (2), Duke University Medical Center, Durham, NC 27710, USA; (3) Baylor College of Medicine, Houston, TX 77030, USA
9:35- 9:50 Talk 3
The Dorsal Mesenchymal Protrusion (DMP), a Second Heart Field Derivative, Plays an Important Role in AV Septal Development.
Snarr, Brian S; O’Neal, Jessica; Chintalapudi, Mastan R; Wirrig, Elaine; Phelps, Aimee; Trusk, Tom; Kubalak, Steven W; Wessels, Andy
Department of Cell Biology and Anatomy, Medical University of South Carolina
9:50- 10:05 Talk 4
Regulation and Function of miR-143 and miR-145 in Heart and Smooth Muscle
Kimberly R. Cordes, Sarah U. Morton, Kathryn N. Ivey, and Deepak Srivastava, Gladstone Institute of Cardiovascular Disease, and Departments of Biochemistry & Biophysics and Pediatrics, University of California San Francisco, 1650 Owens Street, San Francisco, California 94158, USA.
10:05- 10:20 Talk 5
Tbx1 is expressed in multi-potentcardiac progenitor cells and it regulates their proliferation and differentiation
Li Chen1-2, Susan Tang2, Antonio Baldini1-3
1 Program in Cardiovascular Sciences, Baylor College of Medicine, Houston, TX 77030.
2 Institute of Biosciences and Technology, Texas A&M University Health Sciences Center, Houston, TX 77030.
3 University Federico II, and Telethon Institute of Genetics and Medicine, Naples, Italy
10:20- 10:35 AM: Break
10:35-11:55 AM: Platform Session V – Cardiac Conduction System Development
(Chairs: ) (AB Ballroom)
10:35- 10:40 Chair’s Introduction
10:40– 10:55 Talk 1
Zebrafish Cardiac Conduction/Rhythm Mutants: Cellular and Genetic Dissection of the Cardiac Electrical System
Neil Chi, Robin Shaw, Lily Jan, and Didier Stainier
University of California, San Francisco; Department of Biochemistry and Biophysics; Division of Cardiology
10:55- 11:10 Talk 2
Tbx2 controls proliferation, working myocardial differentiation and atrioventricular conduction delay
Wim TJ Aanhaanen1, Bastiaan JD Boukens1, Janynke F Brons1, M Sameer Rana1, Vincent Wakker1, Corrie de Gier-de Vries1, Andreas Kispert 2, Antoon FM Moorman1, Vincent M Christoffels1
1 Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
2 Institut für Molekularbiologie, Medizinische Hochschule Hannover, Hannover, Germany
11:10- 11:25 Talk 3
Deficiency of Zic3 affects conduction system development and patterning.
A.M. Haaning1, R.C. Czosek1,2, and S.M. Ware.1Department of Pediatrics, Cincinnati Children’s Hospital and University of Cincinnati College of Medicine1 and Department of Pediatrics, Division of Pediatric Cardiology, Boston Children’s Hospital2.
11:25- 11:40 Talk 4
abnormal cardiac conduction system development and arrhythmias IN BMPR2 hypomorphIC mutants
Hanwei ZHANG, Laura BURGHI, Vivian CHIU, Fuhua CHEN, Emmanuèle C. Délot
UCLA Mattel Children’s Hospital, Cardiovascular Research Laboratories
11:40- 11:55 Talk 5
Mapping of the Cx30.2 minimal enhancer uncovers a critical role for GATA4 in development of the atrioventricular node
Nikhil V. Munshi, Jeff Berry, John McAnally, Svetlana Bezprozvannaya, Joseph A. Hill, Vidu Garg, and Eric N. Olson.
The University of Texas Southwestern Medical Center at Dallas
Department of Molecular Biology 5323 Harry Hines Boulevard
Dallas, Texas 75390
12:00- 1:00 PM: Box Lunch (Liberty)
1:15-2:15 PM: Concurrent Workshops I
Cardiac Progenitors I – Moderator Antonio Baldini (C Ballroom)
1. Ken Chien
2. Sylvia Evans (not confirmed)
3. Eldad Tzahor
4. Antonio Baldini
Micro RNA’s in Cardiac Development - Moderator Bob Schwartz (BTN)
1. Dazhi Wang
2. Deepak Srivastava
3. Bob Schwartz
2:15– 2:40 PM: Break
2:40– 4:00 PM: Platform Session VI – Signaling Pathways in Cardiogenesis
(Chairs: ) (AB Ballroom)
2:40- 2:45 Chair’s Introduction
2:45– 3:00 Talk 1
Blocking Shh signaling in cardiac neural crest-ablated chick embryos rescues outflow tract septation
Mary Redmond Hutson*, Faustina Sackey, Katherine Luney, Margaret L. Kirby
Department or Pediatrics, Duke University Medical Center, Durham, NC
3:00- 3:15 Talk 2
Hedgehog signaling plays a cell-autonomous role in maximizing cardiac developmental potential
Natalie A. Thomas, Marco Koudijs, Fredericus J.M. van Eeden, Alexandra L. Joyner, and Deborah Yelon
Developmental Genetics Program and Department of Cell Biology, Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016 USA
3:15- 3:30 Talk 3
Hedgehog-dependent atrial septum progenitors are required for cardiac septation
Andrew D. Hoffmann, Michael A. Peterson, and Ivan P. Moskowitz
Departments of Pediatrics and Pathology, The University of Chicago, Chicago, IL, 60637, USA
3:30- 3:45 Talk 4
Signaling via the TGF-b type I receptor Alk5 in heart development
Somyoth Sridurongrit1, Robert Schwartz2, Pilar Ruiz-Lozano3 and Vesa Kaartinen1*
1The Saban Research Institute of Childrens Hospital Los Angeles, 2Baylor College of Medicine, Houston, TX 77030, USA, 3The Burnham Institute for Medical Research.
3:45- 4:00 Talk 5
The novel gene Megf8 plays an essential role in left-right patterning and cardiogenesis
Zhen Zhang1*, Bishwanath Chatterjee1*, Deanne Alpert1, Richard Francis1, Cheng Cui1,
Matthew Daniels2, Qing Yu1, Steven Sabol1, Yongli. Bai3, Maxim Koriabine3, Yuko Yoshinaga3, Wendy Schackman4, Pieter J. DeJong3, Len Pennachio4, Kenneth Kramer1, Cecilia W. Lo1z
*Equal contribution 1Laboratory of Developmental Biology 2Electron Microscopy Core
National Heart Lung and Blood Institute National Institutes of Health, Bethesda, MD 20892-1583 3BACPAC Resources Center, Children’s Hospital Oakland Research Institute, Oakland, CA
4Joint Genome Institute, Lawrence Berkeley National Laboratory Berkeley, CA,
4:00- 4:30 PM: Break
4:30- 5:50 PM: Platform Session VII – Valvulogenesis
(Chairs: Katherine Yutzey and Jose Luis de la Pompa) (AB Ballroom)
4:30- 4:35 Chair’s Introduction
4:35– 4:50 Talk 1
Notch1 Represses Bmp-Dependent Aortic Valve Calcification
Vishal Nigam and Deepak Srivastava
Gladstone Institute of Cardiovascular Disease
Departments of Pediatrics (Cardiology) and Biochemistry & Biophysics, University of California San Francisco, San Francisco, CA 94158
4:50- 5:05 Talk 2
A Human alk2 mutation causes atrioventricular septal defects
Kelly A. Smith, Sonja Chocron, Irene C. Joziasse, Maarten van Dinther, , Jasper J. van der Smagt, Peter A. Doevendans, Peter ten Dijke, Barbara J. Mulder, Jeroen Bakkers
Hubrecht Institute for Developmental Biology and Stem Cell Research and University
Medical Center Utrecht, Utrecht, Netherlands.
5:05- 5:20 Talk 3
Valve Development in Mice Requires GATA4/FOG Transcriptional Complex
Nikolay Manuylov and Sergei Tevosian
Department of Genetics, Dartmouth Medical School, Hanover, NH 03755
5:20- 5:35 Talk 4
BMP-2 Signals EMT via the Type III TGFβ Receptor (TGFβR3)
Todd A. Townsend1, Kellye C. Kirkbride2, Gerard C. Blobe2, and Joey V. Barnett1
1Department of Pharmacology, Vanderbilt University, Nashville, TN
2Department of Medicine, Duke University, Durham, NC
5:35- 5:50 Talk 5
Scleraxis is required for cell lineage differentiation and extracellular matrix remodeling during murine heart valve formation in vivo.
Agata K. Levay1, Jacqueline D. Peacock1, Yinhui Lu2, Robert B. Hinton Jr.3, Karl E. Kadler2, Ronen Schweitzer4, 5 and Joy Lincoln1
1Department of Molecular and Cellular Pharmacology, Leonard M. Miller School of Medicine, University of Miami, 1600 NW 10th Avenue, Miami, FL, 33101, USA
2Wellcome Trust Center for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK
3Division of Molecular and Cardiovascular Biology, Cincinnati Children’s Hospital, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA
4Department of Cell and Developmental Biology, Oregon Health and Science University,
Portland, OR, USA 5Shriner’s Hospital for Children, Research Division, Portland, OR 97239, USA
Dinner on Own
6:00- 8:00 PM: Poster Session II (odd numbered posters are manned)
Saturday May 17th 2008
7:30- 8:45 AM: Continental Breakfast (Lamar)
8:45- 10:05 AM: Platform Session VIII – Stem Cells and Reprogramming
(Chair: Antonio Baldini)
8:45- 8:50 Chair’s Introduction
8:50– 9:05 Talk 1
Normal Commitment but Impaired Maturation of Cardiac Progenitor Cells from Induced Pluripotent Stem Cells
Sean M. Wu,1,8#* Matthias Stadtfeld,2# Min Zeng,1# Esther Yu,1 Yuko Fujiwara,4 Guangwen Wang,5 Stuart H. Orkin,4,6,7,8 Laurie Jackson-Grusby,5,8 Konrad Hochedlinger2,3,8*
1Cardiovascular Research Center, Division of Cardiology, Department of Medicine, 2Center for Regenerative Medicine, 3Cancer Center, Massachusetts General Hospital, Boston, MA 02114. 4Division of Hematology and Oncology, 5Department of Pathology, 6Howard Hughes Medical Institute, Children’s Hospital, Boston, MA 02115, USA. 7Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston MA, 02115, USA. 8Harvard Stem Cell Institute, Cambridge, MA 02138, USA
9:05- 9:20 Talk 2
Enhancing epicardial stem cell repair mechanisms with synthetic small-molecules
Jamie Russell, Sean Goetsch, Houman Khalili, Jenny Hsieh, Hesham Sadek, Jay Schneider.
Depts. of Medicine and Molecular Biology, UT Southwestern Medical Center, Dallas, TX.
9:20- 9:35 Talk 3
Non-invasive in vivo cellular and molecular imaging of transplanted stem cells for cardiac repair/regeneration strategies using magnetic resonance and bioluminescence modalities
David Taylor1, Ramanakumar Kammili1, Kellie Thompson1, Brent French2, and Steven Ebert1*
1Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 2Dept. of Biomedical Engineering, University of Virginia Health System, Charlottesville, VA *Presenting author
9:35- 9:50 Talk 4
Epicardial-derived Wt1+ progenitors contribute to the cardiomyocyte lineage in the developing heart
Bin Zhou1,2, Qing Ma1,2, Satish Rajagopal1,2, Sean M. Wu3, Ibrahim Domian3, José Rivera-Feliciano2, Dawei Jiang1, Alexander von Gise1,2, Sadakatsu Ikeda1,2, Kenneth R. Chien3, and WilliamT. Pu1,2
1Department of Cardiology, Children’s Hospital Boston, 300 Longwood Ave, Boston, MA 02115
2Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 0360, Boston, MA 02115 3Harvard Stem Cell Institute, Harvard University and Cardiovascular Research Center, Massachusetts General Hospital, 185 Cambridge St., Boston, MA 02114
9:50- 10:05 Talk 5
A myocardial lineage derives from Tbx18 epicardial cells
Chen-Leng Cai1,2,3,4*, Jody C. Martin5*, Yunfu Sun1*, Li Cui1, Lianchun Wang6, Kunfu Ouyang7, Lei Yang4, Lei Bu1, Xingqun Liang7, Xiaoxue Zhang1, William B. Stallcup8, Christopher P. Denton9, Andrew McCulloch5, Ju Chen7 & Sylvia M. Evans1
1School of Medicine and Skaggs School of Pharmacy, University of California, San Diego, La Jolla, CA 92093, USA. 2Department of Developmental and Regenerative Biology, 3Center for Molecular Cardiology, 4The Black Family Stem Cell Institute, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA. 5Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA. 6Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA. 7Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA. 8Developmental Neurobiology Program, The Burnham Institute, La Jolla, CA 92037, USA. 9Center for Rheumatology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK.
10:05- 10:15 AM: Break